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Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue
This protocol describes how biological samples, like brain tissue, can be imaged in three dimensions using the focussed ion beam/scanning electron microscope (FIB/SEM). The samples are fixed with aldehydes, heavy metal stained using osmium tetroxide and uranyl acetate. They are then dehydrated with...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3196160/ https://ncbi.nlm.nih.gov/pubmed/21775953 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/2588 |
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