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Correlative In Vivo 2 Photon and Focused Ion Beam Scanning Electron Microscopy of Cortical Neurons

Correlating in vivo imaging of neurons and their synaptic connections with electron microscopy combines dynamic and ultrastructural information. Here we describe a semi-automated technique whereby volumes of brain tissue containing axons and dendrites, previously studied in vivo, are subsequently im...

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Detalhes bibliográficos
Main Authors: Maco, Bohumil, Holtmaat, Anthony, Cantoni, Marco, Kreshuk, Anna, Straehle, Christoph N., Hamprecht, Fred A., Knott, Graham W.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3585404/
https://ncbi.nlm.nih.gov/pubmed/23468982
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0057405
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