Automated Detection and Segmentation of Synaptic Contacts in Nearly Isotropic Serial Electron Microscopy Images

We describe a protocol for fully automated detection and segmentation of asymmetric, presumed excitatory, synapses in serial electron microscopy images of the adult mammalian cerebral cortex, taken with the focused ion beam, scanning electron microscope (FIB/SEM). The procedure is based on interacti...

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Detalhes bibliográficos
Main Authors: Kreshuk, Anna, Straehle, Christoph N., Sommer, Christoph, Koethe, Ullrich, Cantoni, Marco, Knott, Graham, Hamprecht, Fred A.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2011
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3198725/
https://ncbi.nlm.nih.gov/pubmed/22031814
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0024899
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