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Spring-Loaded Mechanism of DNA Unwinding by Heptitis C Virus NS3 Helicase
NS3 is an essential helicase for replication of Hepatitis C virus, and is a model enzyme for investigating helicase function. Using single molecule fluorescence analysis, we show that NS3 unwinds DNA in discrete steps of approximately 3-bp. Dwell time analysis indicates that about three hidden steps...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2007
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3565428/ https://ncbi.nlm.nih.gov/pubmed/17656723 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.1144130 |
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