Single-Molecule Fluorescence Reveals the Unwinding Stepping Mechanism of Replicative Helicase

Bacteriophage T7 gp4 serves as a model protein for replicative helicases that couples deoxythymidine triphosphate (dTTP) hydrolysis to directional movement and DNA strand separation. We employed single-molecule fluorescence resonance energy transfer methods to resolve steps during DNA unwinding by T...

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Main Authors: Syed, Salman, Pandey, Manjula, Patel, Smita S., Ha, Taekjip
Format: Artigo
Sprog:Inglês
Udgivet: Cell Press 2014
Fag:
Report
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3988844/
https://ncbi.nlm.nih.gov/pubmed/24630993
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.celrep.2014.02.022
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