Single Molecule Studies Reveal Dynamics of DNA Unwinding by the Ring-Shaped T7 Helicase

Helicases are molecular motors that separate DNA strands for efficient replication of genomes. We probed the kinetics of individual ring-shaped T7 helicase molecules as they unwound double-stranded DNA (dsDNA) or translocated on single-stranded DNA (ssDNA). A distinctive DNA sequence dependence was...

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Main Authors: Johnson, Daniel S., Bai, Lu, Smith, Benjamin Y., Patel, Smita S., Wang, Michelle D.
Format: Artigo
Jezik:Inglês
Izdano: 2007
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Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2699903/
https://ncbi.nlm.nih.gov/pubmed/17604719
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cell.2007.04.038
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