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Coupling of DNA unwinding to nucleotide hydrolysis in a ring-shaped helicase
The ring-shaped T7 helicase uses the energy of dTTP hydrolysis to perform the mechanical work of translocation and base pair (bp) separation. We have shown that the unwinding rate of T7 helicase decreases with increasing DNA stability. Here, we show that the dTTPase rate also decreases with increasi...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2008
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2435122/ https://ncbi.nlm.nih.gov/pubmed/18497749 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/emboj.2008.100 |
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