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Switching from single-stranded to double-stranded DNA limits the unwinding processivity of ring-shaped T7 DNA helicase

Phage T7 helicase unwinds double-stranded DNA (dsDNA) by encircling one strand while excluding the complementary strand from its central channel. When T7 helicase translocates on single-stranded DNA (ssDNA), it has kilobase processivity; yet, it is unable to processively unwind linear dsDNA, even 60...

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Autors principals: Jeong, Yong-Joo, Rajagopal, Vaishnavi, Patel, Smita S.
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2013
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3627605/
https://ncbi.nlm.nih.gov/pubmed/23446275
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt133
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