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Switching from single-stranded to double-stranded DNA limits the unwinding processivity of ring-shaped T7 DNA helicase
Phage T7 helicase unwinds double-stranded DNA (dsDNA) by encircling one strand while excluding the complementary strand from its central channel. When T7 helicase translocates on single-stranded DNA (ssDNA), it has kilobase processivity; yet, it is unable to processively unwind linear dsDNA, even 60...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3627605/ https://ncbi.nlm.nih.gov/pubmed/23446275 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt133 |
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