Effector Kinase Coupling Enables High-Throughput Screens for Direct HIV-1 Nef Antagonists with Anti-retroviral Activity

HIV-1 Nef, a critical AIDS progression factor, represents an important target protein for antiretroviral drug discovery. Because Nef lacks intrinsic enzymatic activity, we developed an assay that couples Nef to the activation of Hck, a Src-family member and Nef effector protein. Using this assay, we...

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Bibliographic Details
Main Authors: Emert-Sedlak, Lori A., Narute, Purushottam, Shu, Sherry T., Poe, Jerrod A., Shi, Haibin, Yanamala, Naveena, Alvarado, John Jeff, Lazo, John S., Yeh, Joanne I., Johnston, Paul A., Smithgall, Thomas E.
Format: Artigo
Language:Inglês
Published: 2013
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3559019/
https://ncbi.nlm.nih.gov/pubmed/23352142
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2012.11.005
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