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Fast and ultrasensitive method for quantitating prion infectivity titer

Bioassay by end-point dilution has been employed for decades for routine determination of prion infectivity titer. Here we show that the new Protein Misfolding Cyclic Amplification with Teflon beads (PMCAb) can be used to estimate titers of the misfolded version of the prion protein (PrP(Sc)) with a...

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Detalhes bibliográficos
Main Authors: Makarava, Natallia, Savtchenko, Regina, Alexeeva, Irina, Rohwer, Robert G., Baskakov, Ilia V.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3518416/
https://ncbi.nlm.nih.gov/pubmed/22415832
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms1730
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