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Fusion to a highly charged proteasomal retargeting sequence increases soluble cytoplasmic expression and efficacy of diverse anti-synuclein intrabodies

Intrabodies can be powerful reagents to effect modulation of aberrant intracellular proteins that underlie a range of diseases. However, their cytoplasmic solubility can be limiting. We previously reported that overall charge and hydrophilicity can be combined to provide initial estimates of intrace...

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Détails bibliographiques
Auteurs principaux: Joshi, Shubhada N., Butler, David C., Messer, Anne
Format: Artigo
Langue:Inglês
Publié: Landes Bioscience 2012
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3502235/
https://ncbi.nlm.nih.gov/pubmed/22929188
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/mabs.21696
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