Thioredoxin, the Processivity Factor, Sequesters an Exposed Cysteine in the Thumb Domain of Bacteriophage T7 DNA Polymerase

Gene 5 protein (gp5) of bacteriophage T7 is a non-processive DNA polymerase. It achieves processivity by binding to Escherichia coli thioredoxin (trx). gp5/trx complex binds tightly to a primer-DNA template enabling the polymerization of hundreds of nucleotides per binding event. gp5 contains 10 cys...

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Bibliographic Details
Main Authors: Tran, Ngoc Q., Lee, Seung-Joo, Akabayov, Barak, Johnson, Donald E., Richardson, Charles C.
Format: Artigo
Language:Inglês
Published: American Society for Biochemistry and Molecular Biology 2012
Subjects:
Enzymology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3501039/
https://ncbi.nlm.nih.gov/pubmed/23012374
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M112.409235
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