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The loop position of shRNAs and pre-miRNAs is critical for the accuracy of Dicer processing in vivo

Short-hairpin RNA (shRNA)-induced RNAi is used for biological discovery and therapeutics. Dicer, whose normal role is to liberate endogenous miRNAs from their precursors, processes shRNAs into different biologically active siRNAs, affecting their efficacy and potential for off-targeting. We found th...

Πλήρης περιγραφή

Αποθηκεύτηκε σε:
Λεπτομέρειες βιβλιογραφικής εγγραφής
Κύριοι συγγραφείς: Gu, Shuo, Jin, Lan, Zhang, Yue, Huang, Yong, Zhang, Feijie, Valdmanis, Paul N., Kay, Mark A.
Μορφή: Artigo
Γλώσσα:Inglês
Έκδοση: 2012
Θέματα:
Διαθέσιμο Online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3499986/
https://ncbi.nlm.nih.gov/pubmed/23141545
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cell.2012.09.042
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