The loop position of shRNAs and pre-miRNAs is critical for the accuracy of Dicer processing in vivo

Short-hairpin RNA (shRNA)-induced RNAi is used for biological discovery and therapeutics. Dicer, whose normal role is to liberate endogenous miRNAs from their precursors, processes shRNAs into different biologically active siRNAs, affecting their efficacy and potential for off-targeting. We found th...

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Bibliographic Details
Main Authors:	Gu, Shuo, Jin, Lan, Zhang, Yue, Huang, Yong, Zhang, Feijie, Valdmanis, Paul N., Kay, Mark A.
Format:	Artigo
Language:	Inglês
Published:	2012
Subjects:	Article
Online Access:	https://ncbi.nlm.nih.gov/pmc/articles/PMC3499986/ https://ncbi.nlm.nih.gov/pubmed/23141545 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cell.2012.09.042
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The loop position of shRNAs and pre-miRNAs is critical for the accuracy of Dicer processing in vivo

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