First step in pre-miRNAs processing by human Dicer

AIM: To investigate the strand preference of the initial cleavage of human pre-miRNAs by human Dicer in vitro. METHODS: We used a series of in vitro transcribed pre-miRNAs that were radioactively labeled at their 5′ or 3′ ends in cleavage reactions with recombinant human Dicer or HeLa cytoplasmic S1...

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Detalhes bibliográficos
Main Authors: Flores-jasso, Carlos Fabián, Arenas-huertero, Catalina, Reyes, Jose Luis, Contreras-cubas, Cecilia, Covarrubias, Alejandra, Vaca, Luis
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2009
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Original Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4006688/
https://ncbi.nlm.nih.gov/pubmed/19654583
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/aps.2009.108
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