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First step in pre-miRNAs processing by human Dicer
AIM: To investigate the strand preference of the initial cleavage of human pre-miRNAs by human Dicer in vitro. METHODS: We used a series of in vitro transcribed pre-miRNAs that were radioactively labeled at their 5′ or 3′ ends in cleavage reactions with recombinant human Dicer or HeLa cytoplasmic S1...
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| Autors principals: | , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Nature Publishing Group
2009
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4006688/ https://ncbi.nlm.nih.gov/pubmed/19654583 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/aps.2009.108 |
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