Stabilization of Penicillin G Acylase from Escherichia coli: Site-Directed Mutagenesis of the Protein Surface To Increase Multipoint Covalent Attachment

Three mutations on the penicillin acylase surface (increasing the number of Lys in a defined area) were performed. They did not alter the enzyme's stability and kinetic properties; however, after immobilization on glyoxyl-agarose, the mutant enzyme showed improved stability under all tested con...

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Bibliografiske detaljer
Main Authors: Abian, Olga, Grazú, Valeria, Hermoso, Juan, González, Ramón, García, José Luis, Fernández-Lafuente, Roberto, Guisán, José Manuel
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2004
Fag:
Enzymology and Protein Engineering
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC348938/
https://ncbi.nlm.nih.gov/pubmed/14766616
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.2.1249-1251.2004
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