Improving the Activity and Stability of GL-7-ACA Acylase CA130 by Site-Directed Mutagenesis

In the present study, glutaryl-7-amino cephalosporanic acid acylase from Pseudomonas sp. strain 130 (CA130) was mutated to improve its enzymatic activity and stability. Based on the crystal structure of CA130, two series of amino acid residues, one from those directly involved in catalytic function...

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Bibliographische Detailangaben
Hauptverfasser: Zhang, Wei, Liu, Yuan, Zheng, Huabao, Yang, Sheng, Jiang, Weihong
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2005
Schlagworte:
Enzymology and Protein Engineering
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1214626/
https://ncbi.nlm.nih.gov/pubmed/16151116
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.9.5290-5296.2005
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