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Guiding the Design of Synthetic DNA-Binding Molecules with Massively Parallel Sequencing

[Image: see text] Genomic applications of DNA-binding molecules require an unbiased knowledge of their high affinity sites. We report the high-throughput analysis of pyrrole-imidazole polyamide DNA-binding specificity in a 10(12)-member DNA sequence library using affinity purification coupled with m...

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Detalhes bibliográficos
Main Authors: Meier, Jordan L., Yu, Abigail S., Korf, Ian, Segal, David J., Dervan, Peter B.
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2012
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3483022/
https://ncbi.nlm.nih.gov/pubmed/23013524
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja308888c
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