Automation of large scale transient protein expression in mammalian cells

Traditional mammalian expression systems rely on the time-consuming generation of stable cell lines; this is difficult to accommodate within a modern structural biology pipeline. Transient transfections are a fast, cost-effective solution, but require skilled cell culture scientists, making man-powe...

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Main Authors: Zhao, Yuguang, Bishop, Benjamin, Clay, Jordan E., Lu, Weixian, Jones, Margaret, Daenke, Susan, Siebold, Christian, Stuart, David I., Yvonne Jones, E., Radu Aricescu, A.
Format: Artigo
Jezik:Inglês
Izdano: Academic Press 2011
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Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3477309/
https://ncbi.nlm.nih.gov/pubmed/21571074
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2011.04.017
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