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Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins
Structural, biochemical and biophysical studies of eukaryotic soluble and membrane proteins require their production to milligram quantities. Although large-scale protein expression strategies based on transient or stable transfection of mammalian cells are well established, they are associated with...
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| Publicado no: | Nat Protoc |
|---|---|
| Main Authors: | , , , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6364805/ https://ncbi.nlm.nih.gov/pubmed/30455477 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-018-0075-9 |
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