Automating Single Subunit Counting of Membrane Proteins in Mammalian Cells

Elucidating subunit stoichiometry of neurotransmitter receptors is preferably carried out in a mammalian expression system where the rules of native protein assembly are strictly obeyed. Although successful in Xenopus oocytes, single subunit counting, manually counting photobleaching steps of GFP-ta...

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Autors principals: McGuire, Hugo, Aurousseau, Mark R. P., Bowie, Derek, Blunck, Rikard
Format: Artigo
Idioma:Inglês
Publicat: American Society for Biochemistry and Molecular Biology 2012
Matèries:
Molecular Biophysics
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3476259/
https://ncbi.nlm.nih.gov/pubmed/22930752
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M112.402057
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