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Automating Single Subunit Counting of Membrane Proteins in Mammalian Cells
Elucidating subunit stoichiometry of neurotransmitter receptors is preferably carried out in a mammalian expression system where the rules of native protein assembly are strictly obeyed. Although successful in Xenopus oocytes, single subunit counting, manually counting photobleaching steps of GFP-ta...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Biochemistry and Molecular Biology
2012
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3476259/ https://ncbi.nlm.nih.gov/pubmed/22930752 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M112.402057 |
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