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Simple and efficient purification of Escherichia coli DNA polymerase V: Cofactor requirements for optimal activity and processivity in vitro
Most damage induced mutagenesis in Escherichia coli is dependent upon the [Formula: see text] protein complex, which comprises DNA polymerase V (pol V). Biochemical characterization of pol V has been hindered by the fact that the enzyme is notoriously difficult to purify, largely because overproduce...
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| Autores principales: | , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2012
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3471214/ https://ncbi.nlm.nih.gov/pubmed/22341652 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.dnarep.2012.01.012 |
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