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Escherichia coli UmuC active site mutants: effects on translesion DNA synthesis, mutagenesis and cell survival

Escherichia coli polymerase V (pol V/UmuD'(2)C) is a low-fidelity DNA polymerase that has recently been shown to avidly incorporate ribonucleotides (rNTPs) into undamaged DNA. The fidelity and sugar selectivity of pol V can be modified by missense mutations around the “steric gate” of UmuC. Her...

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Detalhes bibliográficos
Main Authors: Kuban, Wojciech, Vaisman, Alexandra, McDonald, John P., Karata, Kiyonobu, Yang, Wei, Goodman, Myron F., Woodgate, Roger
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3419331/
https://ncbi.nlm.nih.gov/pubmed/22784977
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.dnarep.2012.06.005
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