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N-Terminal T4 Lysozyme Fusion Facilitates Crystallization of a G Protein Coupled Receptor

A highly crystallizable T4 lysozyme (T4L) was fused to the N-terminus of the β(2) adrenergic receptor (β(2)AR), a G-protein coupled receptor (GPCR) for catecholamines. We demonstrate that the N-terminal fused T4L is sufficiently rigid relative to the receptor to facilitate crystallogenesis without t...

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Autori principali: Zou, Yaozhong, Weis, William I., Kobilka, Brian K.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3464249/
https://ncbi.nlm.nih.gov/pubmed/23056231
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0046039
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