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Genome-scale promoter engineering by Co-Selection MAGE
Multiplex Automated Genome Engineering (MAGE) employs short oligonucleotides to scarlessly modify genomes. However, insertions of >10 bases are still inefficient, but can be improved substantially by selection of highly modified chromosomes. Here, we describe Co-Selection MAGE (CoS-MAGE) to optim...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3428217/ https://ncbi.nlm.nih.gov/pubmed/22484848 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1971 |
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