Repetitive genomic insertion of gene-sized dsDNAs by targeting the promoter region of a counter-selectable marker

Genome engineering can be used to produce bacterial strains with a wide range of desired phenotypes. However, the incorporation of gene-sized DNA fragments is often challenging due to the intricacy of the procedure, off-target effects, and low insertion efficiency. Here we report a genome engineerin...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Jeong, Jaehwan, Seo, Han Na, Jung, Yu Kyung, Lee, Jeewon, Ryu, Gyuri, Lee, Wookjae, Kwon, Euijin, Ryoo, Keunsoo, Kim, Jungyeon, Cho, Hwa-Young, Cho, Kwang Myung, Park, Jin Hwan, Bang, Duhee
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4348660/
https://ncbi.nlm.nih.gov/pubmed/25736821
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep08712
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