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Repetitive genomic insertion of gene-sized dsDNAs by targeting the promoter region of a counter-selectable marker
Genome engineering can be used to produce bacterial strains with a wide range of desired phenotypes. However, the incorporation of gene-sized DNA fragments is often challenging due to the intricacy of the procedure, off-target effects, and low insertion efficiency. Here we report a genome engineerin...
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| 出版年: | Sci Rep |
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| 主要な著者: | , , , , , , , , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Nature Publishing Group
2015
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4348660/ https://ncbi.nlm.nih.gov/pubmed/25736821 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep08712 |
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