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Synthesis of the human insulin gene. Part II. Further improvements in the modified phosphotriester method and the synthesis of seventeen deoxyribooligonucleotide fragments constituting human insulin chains B and mini-CDNA.

The purification of protected deoxyribooligonucleotides containing phosphotriester internucleotidic linkages has been improved by developing a deactivated silica gel chromatographic technique. The efficiency of this technique as applied in the modified phosphotriester approach has been demonstrated...

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Salvato in:
Dettagli Bibliografici
Pubblicato in:Nucleic Acids Res
Autori principali: Sung, W L, Hsiung, H M, Brousseau, R, Michniewicz, J, Wu, R, Narang, S A
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 1979
Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342380/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/230464/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/7.8.2199
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