Synthesis of the human insulin gene. Part II. Further improvements in the modified phosphotriester method and the synthesis of seventeen deoxyribooligonucleotide fragments constituting human insulin chains B and mini-CDNA.
The purification of protected deoxyribooligonucleotides containing phosphotriester internucleotidic linkages has been improved by developing a deactivated silica gel chromatographic technique. The efficiency of this technique as applied in the modified phosphotriester approach has been demonstrated...
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| Udgivet i: | Nucleic Acids Res |
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| Principais autores: | , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1979
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| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC342380/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/230464/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/7.8.2199 |
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