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Synthesis of the human insulin gene. Part II. Further improvements in the modified phosphotriester method and the synthesis of seventeen deoxyribooligonucleotide fragments constituting human insulin chains B and mini-CDNA.

The purification of protected deoxyribooligonucleotides containing phosphotriester internucleotidic linkages has been improved by developing a deactivated silica gel chromatographic technique. The efficiency of this technique as applied in the modified phosphotriester approach has been demonstrated...

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Bibliographic Details
Main Authors: Sung, W L, Hsiung, H M, Brousseau, R, Michniewicz, J, Wu, R, Narang, S A
Format: Artigo
Language:Inglês
Published: 1979
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC342380/
https://ncbi.nlm.nih.gov/pubmed/230464
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