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Synthesis of human insulin gene. Part I. Development of reversed-phase chromatography in the modified triester method. Its application in the rapid and efficient synthesis of eight deoxyribooligonucleotides fragments constituting human insulin A DNA.

Preparative reversed-phase thin layer chromatography on silanized silica-gel (RP-2 and RP-18) has been developed to purify triester deoxyribooligonucleotides prepared by the modified triester method. The effectiveness of this technique has been demonstrated in the rapid synthesis of eight pure deoxy...

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Dettagli Bibliografici
Autori principali: Hsiung, H M, Brousseau, R, Michniewicz, J, Narang, S A
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1979
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC327778/
https://ncbi.nlm.nih.gov/pubmed/450699
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