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Computational thermostabilization of an enzyme

The thermostabilization of an enzyme while maintaining its activity for industrial or biomedical applications using traditional selection methods can be difficult. We demonstrate a rapid computational approach that identified three mutations within a model enzyme that produce a 10°C increase in appa...

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Autors principals: Korkegian, Aaron, Black, Margaret E., Baker, David, Stoddard, Barry L.
Format: Artigo
Idioma:Inglês
Publicat: 2005
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3412875/
https://ncbi.nlm.nih.gov/pubmed/15879217
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.1107387
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