Oligonucleotide-directed mutagenesis by microscale 'shot-gun' gene synthesis.
We describe a rapid and efficient microscale method for in vitro site-directed mutagenesis by gene synthesis. Mutants are constructed by "shot-gun ligation" of overlapping synthetic oligonucleotides yielding double stranded synthetic DNA of more than 120 nucleotides in length. The terminal oligonucl...
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| Yayımlandı: | Nucleic Acids Res |
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| Asıl Yazarlar: | , , , , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Oxford University Press
1985
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| Online Erişim: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC341236/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3889852/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.9.3305 |
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