Oligonucleotide-directed mutagenesis by microscale 'shot-gun' gene synthesis.

We describe a rapid and efficient microscale method for in vitro site-directed mutagenesis by gene synthesis. Mutants are constructed by "shot-gun ligation" of overlapping synthetic oligonucleotides yielding double stranded synthetic DNA of more than 120 nucleotides in length. The terminal oligonucl...

Descrizione completa

Dettagli Bibliografici

Pubblicato in:	Nucleic Acids Res
Autori principali:	Grundström, T, Zenke, W M, Wintzerith, M, Matthes, H W, Staub, A, Chambon, P
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1985
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC341236/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3889852/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.9.3305
Tags:	Aggiungi Tag Nessun Tag, puoi essere il primo ad aggiungerne!!