Oligonucleotide-directed mutagenesis by microscale 'shot-gun' gene synthesis.

We describe a rapid and efficient microscale method for in vitro site-directed mutagenesis by gene synthesis. Mutants are constructed by "shot-gun ligation" of overlapping synthetic oligonucleotides yielding double stranded synthetic DNA of more than 120 nucleotides in length. The terminal oligonucl...

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Bibliografske podrobnosti

izdano v:	Nucleic Acids Res
Principais autores:	Grundström, T, Zenke, W M, Wintzerith, M, Matthes, H W, Staub, A, Chambon, P
Format:	Artigo
Jezik:	Inglês
Izdano:	Oxford University Press 1985
Online dostop:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC341236/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3889852/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.9.3305
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