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Oligonucleotide-directed mutagenesis by microscale 'shot-gun' gene synthesis.

We describe a rapid and efficient microscale method for in vitro site-directed mutagenesis by gene synthesis. Mutants are constructed by "shot-gun ligation" of overlapping synthetic oligonucleotides yielding double stranded synthetic DNA of more than 120 nucleotides in length. The terminal...

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Publicat a:Nucleic Acids Res
Autors principals: Grundström, T, Zenke, W M, Wintzerith, M, Matthes, H W, Staub, A, Chambon, P
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 1985
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC341236/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3889852/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.9.3305
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