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The final deprotection step in oligonucleotide synthesis is reduced to a mild and rapid ammonia treatment by using labile base-protecting groups.

Phenoxyacetyl (pac) and methoxyacetyl (mac) for adenine and guanine, isobutyryl for cytosine, were successfully applied as amino protecting groups both in phosphotriester and phosphoramidite approaches. As shown by N.M.R. and H.P.L.C. analysis, they are completely deblocked in less than four hours i...

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Λεπτομέρειες βιβλιογραφικής εγγραφής
Κύριοι συγγραφείς: Schulhof, J C, Molko, D, Teoule, R
Μορφή: Artigo
Γλώσσα:Inglês
Έκδοση: 1987
Διαθέσιμο Online:https://ncbi.nlm.nih.gov/pmc/articles/PMC340442/
https://ncbi.nlm.nih.gov/pubmed/3822812
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