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Chemical synthesis of RNA using fast oligonucleotide deprotection chemistry.
The exocyclic amine protecting groups in oligonucleotide synthesis which require 8-16 hours at 55 degrees C for deprotection in ammonia have been replaced with more labile base protecting groups (dimethylformamidine for adenine and guanine and isobutyryl for cytosine). Using these fast oligonucleoti...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1992
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC312168/ https://ncbi.nlm.nih.gov/pubmed/1373236 |
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