Chemical synthesis of RNA using fast oligonucleotide deprotection chemistry.
The exocyclic amine protecting groups in oligonucleotide synthesis which require 8-16 hours at 55 degrees C for deprotection in ammonia have been replaced with more labile base protecting groups (dimethylformamidine for adenine and guanine and isobutyryl for cytosine). Using these fast oligonucleoti...
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| Udgivet i: | Nucleic Acids Res |
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| Principais autores: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1992
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| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC312168/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1373236/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.6.1265 |
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