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Ultraviolet Shadowing of RNA Can Cause Significant Chemical Damage in Seconds

Chemical purity of RNA samples is important for high-precision studies of RNA folding and catalytic behavior, but photodamage accrued during ultraviolet (UV) shadowing steps of sample preparation can reduce this purity. Here, we report the quantitation of UV-induced damage by using reverse transcrip...

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Detalhes bibliográficos
Main Authors: Kladwang, Wipapat, Hum, Justine, Das, Rhiju
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3399121/
https://ncbi.nlm.nih.gov/pubmed/22816040
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep00517
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