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Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp1
Enpp1 is an extracellular membrane-bound glycoprotein that regulates bone mineralization by hydrolyzing ATP to generate pyrophosphate. The extracellular region of mouse Enpp1 was expressed in HEK293S GnT1(−) cells, purified using the TARGET tag/P20.1-Sepharose system and crystallized. An X-ray diff...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
International Union of Crystallography
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3388920/ https://ncbi.nlm.nih.gov/pubmed/22750863 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112019306 |
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