Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Zucchini from Drosophila melanogaster

PIWI-interacting RNAs (piRNAs) bind PIWI proteins and silence transposons to maintain the genomic integrity of germ cells. Zucchini (Zuc), a phospholipase D superfamily member, is conserved among animals and is implicated in piRNA biogenesis. However, the underlying mechanism by which Zuc participat...

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Bibliographic Details
Main Authors: Fukuhara, Satoshi, Nishimasu, Hiroshi, Bonnefond, Luc, Matsumoto, Naoki, Ishitani, Ryuichiro, Nureki, Osamu
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2012
Subjects:
Crystallization Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3515378/
https://ncbi.nlm.nih.gov/pubmed/23143246
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112038936
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