Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp1

Enpp1 is an extracellular membrane-bound glycoprotein that regulates bone mineralization by hydrolyzing ATP to generate pyrophosphate. The extracellular region of mouse Enpp1 was expressed in HEK293S GnT1(−) cells, purified using the TARGET tag/P20.1-Sepharose system and crystallized. An X-­ray diff...

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Detaylı Bibliyografya
Asıl Yazarlar: Kato, Kazuki, Nishimasu, Hiroshi, Mihara, Emiko, Ishitani, Ryuichiro, Takagi, Junichi, Aoki, Junken, Nureki, Osamu
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: International Union of Crystallography 2012
Konular:
Crystallization Communications
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3388920/
https://ncbi.nlm.nih.gov/pubmed/22750863
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309112019306
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