Expressing the human proteome for affinity proteomics: optimising expression of soluble protein domains and in vivo biotinylation

The generation of affinity reagents to large numbers of human proteins depends on the ability to express the target proteins as high-quality antigens. The Structural Genomics Consortium (SGC) focuses on the production and structure determination of human proteins. In a 7-year period, the SGC has dep...

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Detalhes bibliográficos
Main Authors: Keates, Tracy, Cooper, Christopher D.O., Savitsky, Pavel, Allerston, Charles K., Phillips, Claire, Hammarström, Martin, Daga, Neha, Berridge, Georgina, Mahajan, Pravin, Burgess-Brown, Nicola A., Müller, Susanne, Gräslund, Susanne, Gileadi, Opher
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2012
Assuntos:
Research Paper
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3383991/
https://ncbi.nlm.nih.gov/pubmed/22027370
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.nbt.2011.10.007
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