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Lentiviral Fluorescent Protein Expression Vectors for Biotinylation Proteomics
In vivo biotinylation tagging, based on a method in which a protein of interest is tagged with a peptide that is biotinylated in vivo by coexpression of E.coli BirA biotin ligase, has been successfully used for isolation of protein-protein and protein-DNA complexes in mammalian cells. We describe a...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3279158/ https://ncbi.nlm.nih.gov/pubmed/21116996 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61737-950-5_21 |
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