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High-throughput production of human proteins for crystallization: The SGC experience

Producing purified human proteins with high yield and purity remains a considerable challenge. We describe the methods utilized in the Structural Genomics Consortium (SGC) in Oxford, resulting in successful purification of 48% of human proteins attempted; of those, the structures of ∼40% were solved...

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Detalhes bibliográficos
Main Authors: Savitsky, Pavel, Bray, James, Cooper, Christopher D.O., Marsden, Brian D., Mahajan, Pravin, Burgess-Brown, Nicola A., Gileadi, Opher
Formato: Artigo
Idioma:Inglês
Publicado em: Academic Press 2010
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2938586/
https://ncbi.nlm.nih.gov/pubmed/20541610
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2010.06.008
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