The L561A Substitution in the Nascent Base-Pair Binding Pocket of RB69 DNA Polymerase Reduces Base Discrimination

Several variants of RB69 DNA polymerase (RB69 pol) with single-site replacements in the nascent base-pair binding pocket are less discriminating with respect to non-complementary dNMP incorporation than the wild-type enzyme. To quantify the loss in base selectivity, we determined the transient-state...

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Bibliographic Details
Main Authors: Zhang, Hong, Rhee, Chanu, Bebenek, Anna, Drake, John W., Wang, Jimin, Konigsberg, William
Format: Artigo
Language:Inglês
Published: 2006
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3373012/
https://ncbi.nlm.nih.gov/pubmed/16475809
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi052099y
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