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Reversal of a Mutator Activity by a Nearby Fidelity-Neutral Substitution in the RB69 DNA Polymerase Binding Pocket

Phage RB69 family-B DNA polymerase is responsible for the overall high fidelity of RB69 DNA synthesis. Fidelity is compromised when conserved Tyr567, one of the residues that form the nascent polymerase base-pair binding pocket, is replaced by alanine. The Y567A mutator mutant has an enlarged bindin...

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Autori principali: Trzemecka, Anna, Jacewicz, Agata, Carver, Geraldine T., Drake, John W, Bebenek, Anna
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2010
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2991499/
https://ncbi.nlm.nih.gov/pubmed/20950625
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2010.09.058
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