Carregant...

RB69 DNA Polymerase Mutants with Expanded Nascent Base-Pair-Binding Pockets Are Highly Efficient but Have Reduced Base Selectivity

We have investigated the effect of systematically enlarging the nascent base-pair-binding pocket (NBP) of a replicative DNA polymerase from bacteriophage RB69 (RB69 pol) on the incorporation efficiency (k(pol)/K(d,app)) for both correct and incorrect dNMPs. Accordingly, we replaced residues L561, Y5...

Descripció completa

Guardat en:
Dades bibliogràfiques
Autors principals: Zhang, Hong, Beckman, Jeff, Wang, Jimin, Konigsberg, William
Format: Artigo
Idioma:Inglês
Publicat: 2009
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2847438/
https://ncbi.nlm.nih.gov/pubmed/19522539
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi900422b
Etiquetes: Afegir etiqueta
Sense etiquetes, Sigues el primer a etiquetar aquest registre!